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KMID : 0361020010440010015
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Korean Journal of Otolaryngology - Head and Neck Surgery
2001 Volume.44 No. 1 p.15 ~ p.20
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Expression of Voltage-dependent Na+ Channels in the Albino Guinea Pig Cochlea
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이승원/Seung Won Lee
이승규/조남순/송태현/이병돈/장혁순/강주원/김연준/Seung Kyu Lee/Nam Soon Cho/Tae Hyung Song/Byung Don Lee/Hyuck Soon Chang/Ju Won Kang/Yeon Joon Kim
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Abstract
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Background and Objectives : The endolymph produced from cochlear lateral wall regulates fluid and maintains positive endocochlear potential. Although many immunohistochemical studies of ion transport enzymes in the cochlear lateral wall have been
reported, their mechanisms are still not completely understood. And there are no reports on the studies of anti-Na+ channels in the cochlea of the guinea pig. The voltage-dependent ion channels are fundamental components of neuronal
activity.
The Na+ channel has a single α subunit with 4 pseudosubunits of 6 transmembrane segments each. Expression of the pore-forming and voltage-sensing α orαl subunit typically leads to the appearance of channels with voltage- and
time-dependent
gating and ion conductance. The purpose of this study is to evaluate the expression of the Na+ channel type Ⅰ and Ⅱ in the cochlea lateral wall. Materials and Methods : We investigated the protein identification by western blot after
homogenization and immunohistochemical localization by FITC to the anti- Na+ channel type Ⅰand Ⅱin the cochlea of the Preyer's positive, white guinea pigs. Results : The results showed that the anti-Na+ channel type Ⅰand
Ⅱ
were
expressed strongly in the intermediate cells of the stria vascularis, and weakly in the stria vascularis. Conclusion : We suggest that there are voltage-dependent Na+ channels in the stria vascularis of cochlea and those functions are
further evaulated physiologically by the patch clamp technique.
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KEYWORD
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